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Date: 28 September 1988 / 29 September 1988
Heats
Quarter-finals
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Florence Griffith Joyner | USA | 21.34 |
| 2 | Grace Jackson | JAM | 21.72 |
| 3 | Heike Drechsler | GDR | 21.95 |
| 4 | Merlene Ottey | JAM | 21.99 |
| 5 | Silke Moeller | GDR | 22.09 |
| 6 | Gwen Torrence | USA | 22.17 |
| 7 | Maia Azrachvili | URS | 22.33 |
| 8 | Galina Maltchougina | URS | 22.42 |
| Semi-finals | |||
| 9 | Mary Onyali | NGR | 22.43 |
| 10 | Katrin Krabbe | GDR | 22.59 |
| 11 | Nadejda Gueorguieva | BUL | 22.67 |
| 11 | Pauline Davis | BAH | 22.67 |
| 13 | Andrea Thomas | FRG | 22.91 |
| 14 | Paula Dunn | GBR | 23.14 |
| 15 | Agnieszka Siwek | POL | 23.20 |
| 16 | Regula Aebi | SUI | 23.33 |
| Quarter-finals | |||
| 17 | Falilat Ogunkoya | NGR | 22.88 |
| 18 | Kerry Johnson | AUS | 23.01 |
| 19 | Silke Knoll | FRG | 23.15 |
| 20 | Muriel Leroy | FRA | 23.22 |
| 21 | Rita Angotzi | ITA | 23.33 |
| 22 | Jolanta Janota | POL | 23.34 |
| 23 | Simmone Jacobs | GBR | 23.38 |
| 24 | Angela Williams | TTO | 23.48 |
| 25 | Marisa Masullo | ITA | 23.52 |
| 26 | Louise Stuart | GBR | 23.59 |
| 26 | Joycelyn Joseph | ANT | 23.59 |
| 28 | Marie-Christine Cazier-Ballo | FRA | 23.63 |
| 29 | Maria Magnolia Figueiredo | BRA | 23.67 |
| 30 | Karin Janke | FRG | 23.87 |
| 31 | Norfalia Carabali | COL | 23.96 |
| 32 | Marie-Jose Perec | FRA | 24.22 |
| Heats | |||
| 33 | Yolande Straughn | BAR | 23.81 |
| 34 | Ximena Restrepo | COL | 24.00 |
| 35 | Xie Zhiling | CHN | 24.01 |
| 36 | Marina Skordi | GRE | 24.06 |
| 37 | Zhang Xiaoqiong | CHN | 24.08 |
| 38 | Joyce Odhiambo | KEN | 24.26 |
| 39 | Oliver Acii | UGA | 24.39 |
| 40 | Gaily Dube | ZIM | 24.42 |
| 41 | Claudia Acerenza | URU | 24.46 |
| 42 | Ruth Morris | ISV | 24.51 |
| 43 | Agnes Griffith | GRN | 24.79 |
| 44 | Yvonne Hasler | LIE | 24.91 |
| 45 | Woo Yang-Ja | KOR | 24.94 |
| 46 | Ivette Bonapart | SUR | 24.95 |
| 47 | Chen Ya-Li | TPE | 25.03 |
| 48 | Judith Diankolela-Missengui | CGO | 25.20 |
| 49 | Ka Yi Ng | HKG | 25.35 |
| 50 | Marie Felicite Bada | BEN | 25.42 |
| 51 | Melvina Wulah | LBR | 25.46 |
| 52 | Guilhermina da Cruz | ANG | 25.62 |
| 53 | Evelyn Farrell | ARU | 25.74 |
| 54 | Aminata Diarra | MLI | 25.81 |
| 55 | Mariama Ouiminga | BUR | 26.08 |
| 56 | Erin Tierney | COK | 26.16 |
| 57 | Olivette Daruhi | VAN | 26.88 |
| 58 | Rosa Mbuamangongo | GEQ | 31.12 |
| Did not finish | |||
| Pamela Marshall | USA | ||
| Did not start | |||
| Maree Holland | AUS | ||