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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Florence Griffith Joyner | USA | 21.56 |
| 2 | Merlene Ottey | JAM | 22.07 |
| 3 | Silke Moeller | GDR | 22.15 |
| 4 | Maia Azrachvili | URS | 22.33 |
| 5 | Mary Onyali | NGR | 22.43 |
| 6 | Katrin Krabbe | GDR | 22.59 |
| 7 | Pauline Davis | BAH | 22.67 |
| 8 | Andrea Thomas | FRG | 22.91 |
| Rank | Name | Nation | Time |
| 1 | Grace Jackson | JAM | 22.13 |
| 2 | Heike Drechsler | GDR | 22.27 |
| 3 | Gwen Torrence | USA | 22.53 |
| 4 | Galina Maltchougina | URS | 22.55 |
| 5 | Nadejda Gueorguieva | BUL | 22.67 |
| 6 | Paula Dunn | GBR | 23.14 |
| 7 | Agnieszka Siwek | POL | 23.20 |
| 8 | Regula Aebi | SUI | 23.33 |