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Date: 17 September 2000 / 17 September 2000 / 18 September 2000
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Megan Quann | USA | 1:07.05 |
| 2 | Leisel Jones | AUS | 1:07.49 |
| 3 | Penny Heyns | RSA | 1:07.55 |
| 4 | Sarah Poewe | RSA | 1:07.85 |
| 5 | Agnes Kovacs | HUN | 1:08.09 |
| 6 | Masami Tanaka | JPN | 1:08.37 |
| 7 | Tarnee White | AUS | 1:09.09 |
| 8 | Sylvia Gerasch | GER | 1:09.86 |
| Semi-finals | |||
| 9 | Brigitte Becue | BEL | 1:09.47 |
| 10 | Christin Petelski | CAN | 1:09.54 |
| 11 | Rhiannon Leier | CAN | 1:09.63 |
| 12 | Qi Hui | CHN | 1:09.81 |
| 13 | Svitlana Bondarenko | UKR | 1:09.84 |
| 14 | Simone Karn | GER | 1:09.85 |
| 15 | Madelon Baans | NED | 1:10.44 |
| 16 | Natasa Kejzar | SLO | 1:10.66 |
| Heats | |||
| 17 | Olga Bakaldina | RUS | 1:10.53 |
| 18 | Staciana Stitts | USA | 1:10.54 |
| 19 | Li Wei | CHN | 1:10.55 |
| 20 | Heidi Earp | GBR | 1:10.56 |
| 21 | Alicja Peczak | POL | 1:10.57 |
| 22 | Emma Igelstroem | SWE | 1:11.09 |
| 23 | Ilkay Dikmen | TUR | 1:11.51 |
| 24 | Elvira Fischer | AUT | 1:11.58 |
| 25 | Byun Hye-Young | KOR | 1:11.64 |
| 26 | Isabel Ceballos | COL | 1:11.90 |
| 27 | Agata Czaplicki | SUI | 1:13.19 |
| 28 | Joscelin Yeo | SIN | 1:13.25 |
| 29 | Emma Robinson | IRL | 1:13.41 |
| 30 | Smiljana Marinovic | CRO | 1:13.49 |
| 31 | Imaday Nunez | CUB | 1:13.91 |
| 32 | Yi Ting Siow | MAS | 1:13.92 |
| 33 | Iris Edda Heimisdottir | ISL | 1:14.07 |
| 34 | Olga Moltchanova | KGZ | 1:14.41 |
| 35 | Jenny Rose Guerrero | PHI | 1:15.14 |
| 36 | Caroline Sin Wing Chiu | HKG | 1:15.87 |
| 37 | Katerine Moreno | BOL | 1:16.15 |
| 38 | Nadia Cruz | ANG | 1:19.57 |
| 39 | Xenia Peni | PNG | 1:19.62 |
| 40 | Mariam Keita | MLI | 1:37.80 |
| 41 | Balkissa Ouhoumoudou | NIG | 1:42.39 |
| Disqualified | |||
| Doli Akhtar | BAN | ||
| Pamela Girimbabazi | RWA | ||
| Did not start | |||
| Junko Isoda | JPN | ||