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Date: 18 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Megan Quann | USA | 1:07.05 |
| 2 | Leisel Jones | AUS | 1:07.49 |
| 3 | Penny Heyns | RSA | 1:07.55 |
| 4 | Sarah Poewe | RSA | 1:07.85 |
| 5 | Agnes Kovacs | HUN | 1:08.09 |
| 6 | Masami Tanaka | JPN | 1:08.37 |
| 7 | Tarnee White | AUS | 1:09.09 |
| 8 | Sylvia Gerasch | GER | 1:09.86 |