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Date: 17 September 2000
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Sarah Poewe | RSA | 1:07.48 |
| 2 | Agnes Kovacs | HUN | 1:07.79 |
| 3 | Penny Heyns | RSA | 1:08.33 |
| 4 | Sylvia Gerasch | GER | 1:09.33 |
| 5 | Christin Petelski | CAN | 1:09.54 |
| 6 | Rhiannon Leier | CAN | 1:09.63 |
| 7 | Simone Karn | GER | 1:09.85 |
| 8 | Madelon Baans | NED | 1:10.44 |
| Rank | Name | Nation | Time |
| 1 | Megan Quann | USA | 1:07.79 |
| 2 | Leisel Jones | AUS | 1:08.03 |
| 3 | Tarnee White | AUS | 1:08.61 |
| 4 | Masami Tanaka | JPN | 1:09.04 |
| 5 | Brigitte Becue | BEL | 1:09.47 |
| 6 | Qi Hui | CHN | 1:09.81 |
| 7 | Svitlana Bondarenko | UKR | 1:09.84 |
| 8 | Natasa Kejzar | SLO | 1:10.66 |