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Date: 18 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Pieter van den Hoogenband | NED | 1:45.35 |
| 2 | Ian Thorpe | AUS | 1:45.83 |
| 3 | Massimiliano Rosolino | ITA | 1:46.65 |
| 4 | Josh Davis | USA | 1:46.73 |
| 5 | Paul Palmer | GBR | 1:47.95 |
| 6 | James Salter | GBR | 1:48.74 |
| 7 | Rick Say | CAN | 1:48.76 |
| 8 | Grant Hackett | AUS | 1:49.46 |