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Date: 15 August 2010 / 15 August 2010 / 16 August 2010
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Rachel Nicol | CAN | 32.06 |
| 2 | Martina Carraro | ITA | 32.44 |
| 3 | Ana de Pinho Rodrigues | POR | 32.49 |
| 4 | Alessandra Marchioro | BRA | 32.60 |
| 5 | Lina Rathsack | GER | 32.79 |
| 6 | Maria Georgia Michalaka | GRE | 32.90 |
| 7 | Tjasa Vozel | SLO | 33.14 |
| 8 | Urte Kazakeviciute | LTU | 33.22 |
| Semi-finals | |||
| 9 | Noora Laukkanen | FIN | 33.22 |
| 10 | Wang Chang | CHN | 33.29 |
| 11 | Tina Meza | SLO | 33.31 |
| 12 | Taryn MacKenzie | RSA | 33.47 |
| 13 | Evghenia Tanasienco | MDA | 33.63 |
| 14 | Yuliya Litvina | KAZ | 33.64 |
| 15 | Xin Jing Cheryl Lim | SIN | 33.65 |
| 16 | Yvette Man-Yi Kong | HKG | 33.69 |
| Heats | |||
| 17 | Aurelia Trnovcova | SVK | 34.08 |
| 18 | Katarina Durdevic | MNE | 35.21 |
| 19 | Brigitte Rasmussen | ISV | 35.77 |
| 20 | Amanda Jia Xin Liew | BRU | 36.51 |
| 21 | Ho Kum Jong | PRK | 37.10 |
| 22 | Mariana Henriques | ANG | 37.65 |
| 23 | Angelika Ouedraogo | BUR | 42.21 |
| Disqualified | |||
| Maimouna Boubacar Badie | NIG | ||
| Did not start | |||
| Kim Hyejin | KOR | ||