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Date: 15 August 2010
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Ana de Pinho Rodrigues | POR | 32.30 |
| 2 | Alessandra Marchioro | BRA | 32.70 |
| 3 | Lina Rathsack | GER | 32.73 |
| 4 | Maria Georgia Michalaka | GRE | 32.96 |
| 5 | Tina Meza | SLO | 33.31 |
| 6 | Taryn MacKenzie | RSA | 33.47 |
| 7 | Evghenia Tanasienco | MDA | 33.63 |
| 8 | Xin Jing Cheryl Lim | SIN | 33.65 |
| Rank | Name | Nation | Time |
| 1 | Rachel Nicol | CAN | 32.13 |
| 2 | Martina Carraro | ITA | 32.23 |
| 3 | Tjasa Vozel | SLO | 32.87 |
| 4 | Urte Kazakeviciute | LTU | 33.05 |
| 5 | Noora Laukkanen | FIN | 33.22 |
| 6 | Wang Chang | CHN | 33.29 |
| 7 | Yuliya Litvina | KAZ | 33.64 |
| 8 | Yvette Man-Yi Kong | HKG | 33.69 |