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Date: 16 August 2008
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Britta Steffen | GER | 24.43 |
| 2 | Marleen Veldhuis | NED | 24.46 |
| 3 | Lisbeth Trickett | AUS | 24.47 |
| 4 | Hinkelien Schreuder | NED | 24.52 |
| 5 | Kara Lynn Joyce | USA | 24.63 |
| 6 | Aleksandra Gerasimenya | BLR | 24.72 |
| 7 | Francesca Halsall | GBR | 24.80 |
| 8 | Malia Metella | FRA | 24.89 |
| Rank | Name | Nation | Time |
| 1 | Dara Torres | USA | 24.27 |
| 2 | Cate Campbell | AUS | 24.42 |
| 3 | Zhu Yingwen | CHN | 24.76 |
| 4 | Jeanette Ottesen | DEN | 24.86 |
| 5 | Li Zhesi | CHN | 24.90 |
| 6 | Therese Alshammar | SWE | 24.96 |
| 7 | Arlene Semeco | VEN | 25.05 |
| 8 | Hanna-Maria Seppala | FIN | 25.19 |