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Date: 16 August 2008
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Michael Phelps | USA | 50.58 |
| 2 | Milorad Cavic | SRB | 50.59 |
| 3 | Andrew Lauterstein | AUS | 51.12 |
| 4 | Ian Crocker | USA | 51.13 |
| 5 | Jason Dunford | KEN | 51.47 |
| 6 | Takuro Fujii | JPN | 51.50 |
| 7 | Andriy Serdinov | UKR | 51.59 |
| 8 | Ryan Pini | PNG | 51.86 |