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Date: 21 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Inge de Bruijn | NED | 53.83 |
| 2 | Therese Alshammar | SWE | 54.33 |
| 3 | Dara Torres | USA | 54.43 |
| 3 | Jenny Thompson | USA | 54.43 |
| 5 | Martina Moravcova | SVK | 54.72 |
| 6 | Helene Muller | RSA | 55.19 |
| 7 | Sumika Minamoto | JPN | 55.53 |
| 8 | Wilma van Rijn | NED | 55.58 |