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Date: 17 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Inge de Bruijn | NED | 56.61 |
| 2 | Martina Moravcova | SVK | 57.97 |
| 3 | Dara Torres | USA | 58.20 |
| 4 | Petria Thomas | AUS | 58.49 |
| 5 | Jenny Thompson | USA | 58.73 |
| 6 | Junko Onishi | JPN | 59.13 |
| 7 | Susie O'Neill | AUS | 59.27 |
| 8 | Diana Mocanu | ROU | 59.43 |