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Date: 23 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Inge de Bruijn | NED | 24.32 |
| 2 | Therese Alshammar | SWE | 24.51 |
| 3 | Dara Torres | USA | 24.63 |
| 4 | Amy van Dyken | USA | 25.04 |
| 5 | Martina Moravcova | SVK | 25.24 |
| 6 | Sandra Voelker | GER | 25.27 |
| 7 | Alison Sheppard | GBR | 25.45 |
| 8 | Sumika Minamoto | JPN | 25.65 |