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Date: 23 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Lenny Krayzelburg; Ed Moses; Ian Crocker; Gary Hall Jr. | USA | 3:33.73 |
| 2 | Matt Welsh; Regan Harrison; Geoffrey Huegill; Michael Klim | AUS | 3:35.27 |
| 3 | Stev Theloke; Jens Kruppa; Thomas Rupprath; Torsten Spanneberg | GER | 3:35.88 |
| 4 | Klaas-Erik Zwering; Marcel Wouda; Joris Keizer; Pieter van den Hoogenband | NED | 3:37.53 |
| 5 | Peter Horvath; Karoly Guttler; Zsolt Gaspar; Attila Zubor | HUN | 3:39.09 |
| 6 | Chris Renaud; Morgan Knabe; Michael Mintenko; Craig Hutchison | CAN | 3:39.88 |
| 7 | Simon Dufour; Hugues Duboscq; Franck Esposito; Frederick Bousquet | FRA | 3:40.02 |
| 8 | Neil Willey; Darren Mew; James Hickman; Sion Brinn | GBR | 3:40.19 |