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Date: 20 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Pieter van den Hoogenband | NED | 48.30 |
| 2 | Alexander Popov | RUS | 48.69 |
| 3 | Gary Hall Jr. | USA | 48.73 |
| 4 | Michael Klim | AUS | 48.74 |
| 5 | Neil Walker | USA | 49.09 |
| 6 | Lars Froelander | SWE | 49.22 |
| 7 | Denis Pimankov | RUS | 49.36 |
| 8 | Chris Fydler | AUS | 49.44 |