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Date: 22 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Lars Froelander | SWE | 52.00 |
| 2 | Michael Klim | AUS | 52.18 |
| 3 | Geoffrey Huegill | AUS | 52.22 |
| 4 | Ian Crocker | USA | 52.44 |
| 5 | Michael Mintenko | CAN | 52.58 |
| 5 | Takashi Yamamoto | JPN | 52.58 |
| 7 | Thomas Rupprath | GER | 53.13 |
| 7 | Anatoli Poliakov | RUS | 53.13 |