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Date: 27 September 2000 / 28 September 2000
Heats
Quarter-finals
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Pauline Davis-Thompson | BAH | 22.27 |
| 2 | Susanthika Jayasinghe | SRI | 22.28 |
| 3 | Beverly McDonald | JAM | 22.35 |
| 4 | Debbie Ferguson | BAH | 22.37 |
| 5 | Melinda Gainsford-Taylor | AUS | 22.42 |
| 6 | Cathy Freeman | AUS | 22.53 |
| 7 | Zhanna Pintusevych | UKR | 22.66 |
| Semi-finals | |||
| 8 | Louise Ayetotche | CIV | 22.76 |
| 9 | Torri Edwards | USA | 23.06 |
| 10 | Felipa Palacios | COL | 23.11 |
| 11 | Muriel Hurtis | FRA | 23.13 |
| 12 | Nanceen Perry | USA | 23.16 |
| 13 | Mercy Nku | NGR | 23.40 |
| 14 | Lauren Hewitt | AUS | 23.44 |
| 15 | Myriam Mani | CMR | 23.47 |
| Quarter-finals | |||
| 16 | Alenka Bikar | SLO | 23.01 |
| 17 | Mary Onyali-Omagbemi | NGR | 23.03 |
| 18 | Marina Trandenkova | RUS | 23.10 |
| 19 | Oxana Ekk | RUS | 23.17 |
| 20 | Samantha Davies | GBR | 23.20 |
| 21 | Chandra Sturrup | BAH | 23.21 |
| 21 | Fatima Yusuf-Olukoju | NGR | 23.21 |
| 23 | Sabrina Mulrain | GER | 23.24 |
| 24 | Irina Khabarova | RUS | 23.27 |
| 25 | Juliet Campbell | JAM | 23.34 |
| 26 | Johanna Manninen | FIN | 23.41 |
| 27 | Joice Maduaka | GBR | 23.57 |
| 27 | Valma Bass | SKN | 23.57 |
| 29 | Olena Pastushenko | UKR | 23.63 |
| 30 | Astia Walker | JAM | |
| 31 | Cydonie Mothersill | CAY | |
| Heats | |||
| 32 | Sarah Reilly | IRL | 23.43 |
| 33 | Mireille Donders | SUI | 23.44 |
| 34 | Aida Diop | SEN | 23.46 |
| 35 | Monica Twum | GHA | 23.51 |
| 36 | Ekaterini Koffa | GRE | 23.53 |
| 37 | Liu Xiaomei | CHN | 23.56 |
| 38 | Zuzanna Radecka | POL | 23.57 |
| 39 | Hellena Wrappah | GHA | 23.64 |
| 40 | Kaltouma Nadjina | CHA | 23.81 |
| 41 | Lyubov Perepelova | UZB | 23.83 |
| 42 | Joanne Durant | BAR | 23.90 |
| 42 | Karin Mayr | AUT | 23.90 |
| 44 | Qin Wangping | CHN | 24.10 |
| 45 | Mila Savic | SCG | 24.12 |
| 46 | Monika Gachevska | BUL | 24.16 |
| 47 | Heather Samuel | ANT | 24.44 |
| 48 | Akonga Nsimbo | COD | 25.35 |
| 49 | Lineo Shoai | LES | 25.57 |
| 50 | Hana Ali Saleh | YEM | 30.36 |
| Disqualified | |||
| Marion Jones | USA | 22.75 | |
| Did not start | |||
| Ameerah Bello | ISV | ||
| Manuela Levorato | ITA | ||
| Andrea Philipp | GER | ||