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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Marie-Jose Perec | FRA | 49.48 |
| 2 | Ximena Restrepo | COL | 49.76 |
| 3 | Olga Bryzgina | EUN | 49.76 |
| 4 | Phylis Smith | GBR | 50.40 |
| 5 | Jearl Miles | USA | 50.57 |
| 6 | Natasha Kaiser | USA | 50.60 |
| 7 | Michelle Lock | AUS | 50.78 |
| 8 | Elena Ruzina | EUN | 51.30 |
| Rank | Name | Nation | Time |
| 1 | Jill Richardson-Briscoe | CAN | 50.02 |
| 2 | Olga Nazarova | EUN | 50.31 |
| 3 | Sandie Richards | JAM | 50.35 |
| 4 | Rochelle Stevens | USA | 50.37 |
| 5 | Norfalia Carabali | COL | 51.75 |
| 6 | Sandra Douglas | GBR | 51.96 |
| 7 | Renee Poetschka | AUS | 52.09 |
| 8 | Elsa Devassoigne | FRA | 52.85 |