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Date: 30 August 1972
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Roland Matthes | GDR | 56.51 |
| 2 | Bruce Robertson | CAN | 56.86 |
| 3 | Byron MacDonald | CAN | 57.06 |
| 4 | Neil Rogers | AUS | 57.28 |
| 5 | Roger Pyttel | GDR | 57.69 |
| 6 | Sergio Waismann | BRA | 58.07 |
| 7 | Lutz Stocklasa | FRG | 58.33 |
| 8 | Geoffrey Ferreira | TTO | 58.54 |
| Rank | Name | Nation | Time |
| 1 | Mark Spitz | USA | 55.98 |
| 2 | Jerry Heidenreich | USA | 56.18 |
| 3 | David Edgar | USA | 56.88 |
| 4 | Hartmut Flockner | GDR | 56.96 |
| 5 | Juan Bello | PER | 57.51 |
| 6 | Robert Kasting | CAN | 57.67 |
| 7 | John Mills | GBR | 58.13 |
| 8 | Istvan Szentirmay | HUN | 58.25 |