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Date: 20 August 2010
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Tang Yi | CHN | 25.40 |
| 1 | Anna Santamans | FRA | 25.40 |
| 3 | Emma McKeon | AUS | 25.61 |
| 4 | Alessandra Marchioro | BRA | 25.92 |
| 5 | Lovisa Eriksson | SWE | 25.96 |
| 6 | Xiang Qi Amanda Lim | SIN | 26.05 |
| 7 | Carolina Bergamaschi | BRA | 26.24 |
| 8 | Manon Minneboo | NED | 26.35 |