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Date: 17 September 2000 / 17 September 2000 / 18 September 2000
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Diana Mocanu | ROU | 1:00.21 |
| 2 | Mai Nakamura | JPN | 1:00.55 |
| 3 | Nina Zhivanevskaya | ESP | 1:00.89 |
| 4 | Roxana Maracineanu | FRA | 1:01.10 |
| 5 | Noriko Inada | JPN | 1:01.14 |
| 6 | B.J. Bedford | USA | 1:01.47 |
| 7 | Dyana Calub | AUS | 1:01.61 |
| 8 | Louise Oernstedt | DEN | 1:02.02 |
| Semi-finals | |||
| 9 | Antje Buschschulte | GER | 1:01.91 |
| 10 | Kelly Stefanyshyn | CAN | 1:02.35 |
| 10 | Katy Sexton | GBR | 1:02.35 |
| 12 | Kirsty Coventry | ZIM | 1:02.54 |
| 13 | Michelle Lischinsky | CAN | 1:02.55 |
| 14 | Zhan Shu | CHN | 1:02.92 |
| 15 | Sandra Voelker | GER | 1:03.01 |
| 16 | Lu Donghua | CHN | 1:03.31 |
| Heats | |||
| 17 | Charlene Wittstock | RSA | 1:03.18 |
| 18 | Courtney Shealy | USA | 1:03.19 |
| 19 | Shim Min-Ji | KOR | 1:03.20 |
| 19 | Giaan Rooney | AUS | 1:03.20 |
| 21 | Sarah Price | GBR | 1:03.22 |
| 22 | Ilona Hlavackova | CZE | 1:03.28 |
| 23 | Anu Koivisto | FIN | 1:03.44 |
| 24 | Fabiola Molina | BRA | 1:03.68 |
| 25 | Aleksandra Miciul | POL | 1:04.51 |
| 26 | Monique Robins | NZL | 1:04.52 |
| 27 | Nadiya Beshevli | UKR | 1:04.66 |
| 27 | Sofie Wolfs | BEL | 1:04.66 |
| 29 | Irina Raevskaia | RUS | 1:04.76 |
| 30 | Ana Maria Gonzalez | CUB | 1:04.95 |
| 31 | Camilla Johansson | SWE | 1:04.99 |
| 32 | Aikaterini Bliamou | GRE | 1:05.09 |
| 33 | Hiu Wai Sherry Tsai | HKG | 1:05.28 |
| 34 | Brenda Starink | NED | 1:05.93 |
| 35 | Chonlathorn Vorathamrong | THA | 1:05.98 |
| 36 | Annamaria Kiss | HUN | 1:06.12 |
| 37 | Marie-Lizza Danila | PHI | 1:06.48 |
| 38 | Elsa Manora Nasution | INA | 1:06.57 |
| 38 | Serrana Fernandez | URU | 1:06.57 |
| 40 | Kuan Chia-Hsien | TPE | 1:07.18 |
| 41 | Marica Strazmester | SCG | 1:07.21 |
| 42 | Sadan Derya Erke | TUR | 1:07.26 |
| 43 | Kolbrun Yr Kristjansdottir | ISL | 1:07.28 |
| 44 | Anjelika Solovieva | KGZ | 1:07.63 |
| 45 | Andrea Prono | PAR | 1:08.11 |
| 46 | Monika Bakale | CGO | 1:16.36 |
| Did not start | |||
| Tessa Solomon | AHO | ||