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Date: 17 September 2000
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Diana Mocanu | ROU | 1:00.70 |
| 2 | B.J. Bedford | USA | 1:01.61 |
| 3 | Louise Oernstedt | DEN | 1:01.69 |
| 4 | Dyana Calub | AUS | 1:01.86 |
| 5 | Kelly Stefanyshyn | CAN | 1:02.35 |
| 6 | Kirsty Coventry | ZIM | 1:02.54 |
| 7 | Michelle Lischinsky | CAN | 1:02.55 |
| 8 | Zhan Shu | CHN | 1:02.92 |
| Rank | Name | Nation | Time |
| 1 | Mai Nakamura | JPN | 1:01.07 |
| 2 | Noriko Inada | JPN | 1:01.25 |
| 3 | Nina Zhivanevskaya | ESP | 1:01.41 |
| 4 | Roxana Maracineanu | FRA | 1:01.61 |
| 5 | Antje Buschschulte | GER | 1:01.91 |
| 6 | Katy Sexton | GBR | 1:02.35 |
| 7 | Sandra Voelker | GER | 1:03.01 |
| 8 | Lu Donghua | CHN | 1:03.31 |