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Date: 18 August 2010
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Mathilde Cini | FRA | 29.22 |
| 2 | Daryna Zevina | UKR | 29.51 |
| 3 | Klaudia Nazieblo | POL | 29.91 |
| 4 | Yekaterina Rudenko | KAZ | 30.09 |
| 5 | Lotta Nevalainen | FIN | 30.22 |
| 6 | Kendese Nangle | JAM | 30.91 |
| 7 | Siona Huxley | LCA | 30.98 |
| 8 | Amel Melih | ALG | 31.82 |
| Rank | Name | Nation | Time |
| 1 | Alexandra Papusha | RUS | 29.55 |
| 2 | Lovisa Eriksson | SWE | 29.82 |
| 3 | Sarah Rolko | LUX | 30.35 |
| 4 | Isabella Arcila | COL | 30.42 |
| 5 | Juanita Barreto | COL | 30.76 |
| 6 | Monica Ramirez Abella | AND | 30.82 |
| 7 | Chen Ting | TPE | 31.03 |
| 8 | Adeline Shu Jian Winata | SIN | 31.48 |