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Date: 17 August 2010 / 17 August 2010 / 18 August 2010
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Liu Lan | CHN | 26.97 |
| 2 | Elena di Liddo | ITA | 27.06 |
| 3 | Anna Santamans | FRA | 27.31 |
| 4 | Katarina Listopadova | SVK | 27.38 |
| 5 | Lindsay Delmar | CAN | 27.54 |
| 6 | Lovisa Eriksson | SWE | 27.55 |
| 7 | Rachael Kelly | GBR | 27.77 |
| 8 | Katja Hajdinjak | SLO | 27.86 |
| Semi-finals | |||
| 9 | Bruna Rocha | BRA | 28.09 |
| 10 | Anna Marti | ESP | 28.25 |
| 11 | Lena Kalla | GER | 28.31 |
| 12 | Zoe Johnson | AUS | 28.43 |
| 13 | Bryndis Run Hansen | ISL | 28.53 |
| 14 | Danielle Villars | SUI | 28.59 |
| 15 | Anna Schegoleva | CYP | 28.80 |
| 16 | Xiang Qi Amanda Lim | SIN | 29.34 |
| Heats | |||
| 17 | Khadeja Phillip | TTO | 30.04 |
| 18 | Kathryn Millin | SWZ | 30.31 |
| 19 | Aina Filsrabetsara | MAD | 30.54 |
| 20 | Sabine Hazboun | PLE | 31.88 |
| 21 | Sonia Akter | BAN | 32.93 |
| 22 | Reni Jani | ALB | 34.43 |
| 23 | Surennyam Erdenebileg | MGL | 36.12 |