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Date: 20 August 2010
Summary
Heat 1
| Rank | Name | Nation | Time |
| 1 | Bai Anqi; Wang Ximing; Sun Bowei; Tang Yi | CHN | 3:56.78 |
| 2 | Adrian Mantas; Teresa Gutierrez; Judit Ignacio; Aitor Martinez | ESP | 4:07.05 |
| 3 | Kin Tat Kent Cheung; Yvette Man-Yi Kong; Ching Tat Lum; Wai Ting Yu | HKG | 4:14.66 |
| 4 | Stefan Sorak; Marija Joksimovic; Katarina Simonovic; Velimir Stjepanovic | SRB | 4:21.02 |
| Rank | Name | Nation | Time |
| 1 | Alexandra Papusha; Alexey Atsapkin; Ilya Lemaev; Ekaterina Andreeva | RUS | 4:00.67 |
| 2 | Marie Jugnet; Thomas Rabeisen; Medhy Metella; Mathilde Cini | FRA | 4:00.82 |
| 3 | Jeremy Bagshaw; Rachel Nicol; Lindsay Delmar; Chad Bobrosky | CAN | 4:02.69 |
| 4 | Kaitlyn Jones; Austin Ringquist; Erich Peske; Jordan Mattern | USA | 4:04.29 |
| 5 | Oneida Cooper; Dylan Bosch; Chad le Clos; Kyla Ferreira | RSA | 4:06.14 |
| 6 | Ingibjorg Kristin Jonsdottir; Hrafn Traustason; Bryndis Run Hansen; Anton Sveinn McKee | ISL | 4:18.81 |
| Rank | Name | Nation | Time |
| 1 | Madison Wilson; Nicholas Schafer; Zoe Johnson; Justin James | AUS | 3:59.14 |
| 2 | Christian Diener; Lina Rathsack; Lena Kalla; Kevin Leithold | GER | 4:02.56 |
| 3 | Yukiko Watanabe; Tatsunari Shoda; Mayuko Okada; Takahiro Tsutsumi | JPN | 4:06.13 |
| 4 | Pedro Antonio Costa; Vinicius Borges; Bruna Rocha; Julia Gerotto | BRA | 4:08.25 |
| 5 | Rainer Kai Wee Ng; Xin Jing Cheryl Lim; Sheng Jun Pang; Adeline Shu Jian Winata | SIN | 4:08.94 |
| 6 | Kimberlee John-Williams; Khadeja Phillip; Cadell Lyons; Christian Homer | TTO | 4:20.21 |