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Date: 12 August 2008
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Natalie Coughlin | USA | 58.96 |
| 2 | Kirsty Coventry | ZIM | 59.19 |
| 3 | Margaret Hoelzer | USA | 59.34 |
| 4 | Gemma Spofforth | GBR | 59.38 |
| 5 | Anastasia Zueva | RUS | 59.40 |
| 6 | Reiko Nakamura | JPN | 59.72 |
| 7 | Laure Manaudou | FRA | 1:00.10 |
| 8 | Hanae Ito | JPN | 1:00.18 |