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Date: 11 August 2008
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Natalie Coughlin | USA | 59.43 |
| 2 | Reiko Nakamura | JPN | 59.64 |
| 3 | Gemma Spofforth | GBR | 59.79 |
| 4 | Hanae Ito | JPN | 1:00.13 |
| 5 | Elizabeth Simmonds | GBR | 1:00.39 |
| 6 | Julia Wilkinson | CAN | 1:00.60 |
| 7 | Sophie Edington | AUS | 1:01.05 |
| 8 | Kseniya Moskvina | RUS | 1:01.06 |
| Rank | Name | Nation | Time |
| 1 | Kirsty Coventry | ZIM | 58.77 |
| 2 | Anastasia Zueva | RUS | 59.77 |
| 3 | Margaret Hoelzer | USA | 59.84 |
| 4 | Laure Manaudou | FRA | 1:00.19 |
| 5 | Emily Seebohm | AUS | 1:00.31 |
| 6 | Nina Zhivanevskaya | ESP | 1:00.50 |
| 7 | Antje Buschschulte | GER | 1:01.15 |
| 8 | Elizabeth Coster | NZL | 1:01.45 |