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Date: 10 August 2008 / 11 August 2008 / 12 August 2008
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Leisel Jones | AUS | 1:05.17 |
| 2 | Rebecca Soni | USA | 1:06.73 |
| 3 | Mirna Jukic | AUT | 1:07.34 |
| 4 | Yuliya Efimova | RUS | 1:07.43 |
| 5 | Megan Jendrick | USA | 1:07.62 |
| 6 | Tarnee White | AUS | 1:07.63 |
| 7 | Sun Ye | CHN | 1:08.08 |
| 8 | Asami Kitagawa | JPN | 1:08.43 |
| Semi-finals | |||
| 9 | Joline Hostman | SWE | 1:08.26 |
| 10 | Annamay Pierse | CAN | 1:08.27 |
| 11 | Kate Haywood | GBR | 1:08.36 |
| 12 | Chen Huijia | CHN | 1:08.60 |
| 13 | Jillian Tyler | CAN | 1:09.00 |
| 13 | Elise Matthysen | BEL | 1:09.00 |
| 15 | Kirsty Balfour | GBR | 1:09.23 |
| 16 | Suzaan van Biljon | RSA | 1:09.56 |
| Heats | |||
| 17 | Megumi Taneda | JPN | 1:08.45 |
| 18 | Elena Bogomazova | RUS | 1:08.63 |
| 19 | Sara Elbekri | MAR | 1:08.66 |
| 20 | Sarah Poewe | GER | 1:08.69 |
| 21 | Hanna Westrin | SWE | 1:08.80 |
| 22 | Roberta Panara | ITA | 1:08.90 |
| 23 | Jung Seulki | KOR | 1:09.26 |
| 24 | Yuliya Pidlisna | UKR | 1:09.72 |
| 25 | Anna Khlistunova | UKR | 1:09.95 |
| 26 | Diana Gomes | POR | 1:10.02 |
| 27 | Inna Kapishina | BLR | 1:10.15 |
| 28 | Dilara Buse Gunaydin | TUR | 1:10.45 |
| 29 | Sophie de Ronchi | FRA | 1:10.46 |
| 30 | Angeliki Exarchou | GRE | 1:10.47 |
| 31 | Adriana Marmolejo | MEX | 1:10.73 |
| 32 | Nicolette Teo | SIN | 1:10.75 |
| 33 | Smiljana Marinovic | CRO | 1:10.94 |
| 34 | Yekaterina Sadovnik | KAZ | 1:11.14 |
| 35 | Jolijn van Valkengoed | NED | 1:11.26 |
| 36 | Sonja Schober | GER | 1:11.36 |
| 37 | Liliana Eugenia Guiscardo | ARG | 1:11.43 |
| 38 | Valeria Silva | PER | 1:11.64 |
| 39 | Tatiane Sakemi | BRA | 1:11.75 |
| 40 | Erla Dogg Haraldsdottir | ISL | 1:11.78 |
| 41 | Reka Pecz | HUN | 1:12.17 |
| 42 | Danielle Beaubrun | LCA | 1:12.85 |
| 43 | Nadja Higl | SRB | 1:13.19 |
| 44 | Mayumi Raheem | SRI | 1:15.33 |
| 45 | Nibal Yamout | LBN | 1:16.17 |
| 46 | Oksana Hatamkhanova | AZE | 1:20.22 |
| 47 | Asmahan Farhat | LBA | 1:21.68 |
| 48 | Ann Salnikova | GEO | 1:21.70 |
| 49 | Mariam Keita | MLI | 1:24.26 |