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Date: 14 August 2008
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Alain Bernard | FRA | 47.21 |
| 2 | Eamon Sullivan | AUS | 47.32 |
| 3 | Jason Lezak | USA | 47.67 |
| 3 | Cesar Cielo Filho | BRA | 47.67 |
| 5 | Pieter van den Hoogenband | NED | 47.75 |
| 6 | Lyndon Ferns | RSA | 48.04 |
| 7 | Matt Targett | AUS | 48.20 |
| 8 | Stefan Nystrand | SWE | 48.33 |