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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Jeremy Wariner | USA | 44.15 |
| 2 | Chris Brown | BAH | 44.59 |
| 3 | Kevin Borlée | BEL | 44.88 |
| 4 | Nery Brenes | CRC | 44.94 |
| 5 | Saul Weigopwa | NGR | 45.02 |
| 6 | Williams Collazo | CUB | 45.06 |
| 7 | Tabarie Henry | ISV | 45.19 |
| 8 | Claudio Licciardello | ITA | 45.64 |
| Rank | Name | Nation | Time |
| 1 | Leslie Djhone | FRA | 44.79 |
| 2 | David Neville | USA | 44.91 |
| 3 | Joel Milburn | AUS | 45.06 |
| 4 | Ricardo Chambers | JAM | 45.09 |
| 5 | Jonathan Borlée | BEL | 45.11 |
| 6 | Godday James | NGR | 45.24 |
| 7 | Andretti Bain | BAH | 45.52 |
| 8 | Andrew Steele | GBR | 45.59 |
| Rank | Name | Nation | Time |
| 1 | LaShawn Merritt | USA | 44.12 |
| 2 | Martyn Rooney | GBR | 44.60 |
| 3 | Johan Wissman | SWE | 44.64 |
| 4 | Renny Quow | TTO | 44.82 |
| 5 | Gary Kikaya | COD | 44.94 |
| 6 | Michael Mathieu | BAH | 45.56 |
| 7 | Sean Wroe | AUS | 45.56 |
| 8 | Cedric van Branteghem | BEL | 45.81 |