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Date: 15 August 2004 / 15 August 2004 / 16 August 2004
Heats
Semi-finals
Final
| Rank | Name | Nation | Time |
| 1 | Natalie Coughlin | USA | 1:00.37 |
| 2 | Kirsty Coventry | ZIM | 1:00.50 |
| 3 | Laure Manaudou | FRA | 1:00.88 |
| 4 | Reiko Nakamura | JPN | 1:01.05 |
| 5 | Nina Zhivanevskaya | ESP | 1:01.12 |
| 6 | Antje Buschschulte | GER | 1:01.39 |
| 7 | Louise Oernstedt | DEN | 1:01.51 |
| 8 | Haley Cope | USA | 1:01.76 |
| Semi-finals | |||
| 9 | Giaan Rooney | AUS | 1:01.41 |
| 10 | Stanislava Komarova | RUS | 1:01.63 |
| 11 | Noriko Inada | JPN | 1:01.74 |
| 12 | Ilona Hlavackova | CZE | 1:01.81 |
| 13 | Katy Sexton | GBR | 1:01.96 |
| 14 | Zhan Shu | CHN | 1:02.10 |
| 15 | Gao Chang | CHN | 1:02.17 |
| 16 | Sarah Price | GBR | 1:02.48 |
| Heats | |||
| 17 | Sanja Jovanovic | CRO | 1:02.47 |
| 18 | Erin Gammel | CAN | 1:02.47 |
| 19 | Iryna Amshennikova | UKR | 1:02.57 |
| 20 | Marieke Guehrer | AUS | 1:02.76 |
| 21 | Nikolett Szepesi | HUN | 1:02.78 |
| 22 | Hannah McLean | NZL | 1:03.09 |
| 23 | Janine Pietsch | GER | 1:03.13 |
| 24 | Shim Min-Ji | KOR | 1:03.14 |
| 25 | Svetlana Khokhlova | BLR | 1:03.25 |
| 26 | Alessandra Cappa | ITA | 1:03.50 |
| 27 | Gisela Morales | GUA | 1:03.72 |
| 28 | Anna Gostomelsky | ISR | 1:04.06 |
| 29 | Alexandra Putra | FRA | 1:04.13 |
| 30 | Hiu Wai Sherry Tsai | HKG | 1:04.25 |
| 31 | Kiera Aitken | BER | 1:04.37 |
| 32 | Chonlathorn Vorathamrong | THA | 1:05.15 |
| 33 | Eirini Karastergiou | GRE | 1:05.30 |
| 34 | Sadan Derya Erke | TUR | 1:05.38 |
| 35 | Serrana Fernandez | URU | 1:05.51 |
| 36 | Hanna-Maria Seppala | FIN | 1:05.55 |
| 37 | Fu Hsiao Han | TPE | 1:06.62 |
| 38 | Anastassiya Prilepa | KAZ | 1:07.55 |
| 39 | Lenient Obia | NGR | 1:09.65 |
| 40 | Ana Galindo | HON | 1:11.80 |
| 41 | Olga Gnedovckaya | UZB | 1:15.33 |
| 42 | Yelena Rojkova | TKM | 1:15.48 |