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Date: 19 August 2004
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Jodie Henry | AUS | 53.84 |
| 2 | Inge de Bruijn | NED | 54.16 |
| 3 | Natalie Coughlin | USA | 54.40 |
| 4 | Malia Metella | FRA | 54.50 |
| 5 | Kara Lynn Joyce | USA | 54.54 |
| 6 | Nery Mantey Niangkouara | GRE | 54.81 |
| 7 | Martina Moravcova | SVK | 55.12 |
| 8 | Alena Popchanka | BLR | 55.24 |