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Date: 18 August 2004
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Kara Lynn Joyce | USA | 54.81 |
| 2 | Alena Popchanka | BLR | 54.97 |
| 3 | Nery Mantey Niangkouara | GRE | 55.02 |
| 4 | Martina Moravcova | SVK | 55.08 |
| 5 | Lisbeth Lenton | AUS | 55.17 |
| 6 | Marleen Veldhuis | NED | 55.32 |
| 7 | Hanna Shcherba | BLR | 55.67 |
| 8 | Ryu Yoon-Ji | KOR | 55.85 |
| Rank | Name | Nation | Time |
| 1 | Jodie Henry | AUS | 53.52 |
| 2 | Inge de Bruijn | NED | 54.06 |
| 3 | Natalie Coughlin | USA | 54.37 |
| 4 | Malia Metella | FRA | 54.57 |
| 5 | Federica Pellegrini | ITA | 55.30 |
| 6 | Hanna-Maria Seppala | FIN | 55.59 |
| 7 | Josefin Lillhage | SWE | 55.76 |
| 8 | Tomoko Nagai | JPN | 56.03 |