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Date: 20 August 2004
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Kara Lynn Joyce | USA | 25.06 |
| 2 | Therese Alshammar | SWE | 25.15 |
| 3 | Marleen Veldhuis | NED | 25.27 |
| 4 | Rebeca Gusmao | BRA | 25.31 |
| 5 | Alison Sheppard | GBR | 25.36 |
| 6 | Eileen Marie Coparropa Aleman | PAN | 25.37 |
| 6 | Cristina Chiuso | ITA | 25.37 |
| 8 | Svetlana Khokhlova | BLR | 25.47 |
| Rank | Name | Nation | Time |
| 1 | Inge de Bruijn | NED | 24.56 |
| 2 | Lisbeth Lenton | AUS | 24.90 |
| 3 | Malia Metella | FRA | 24.99 |
| 4 | Michelle Engelsman | AUS | 25.13 |
| 5 | Jenny Thompson | USA | 25.17 |
| 5 | Flavia Cazziolato | BRA | 25.17 |
| 7 | Nery Mantey Niangkouara | GRE | 25.27 |
| 8 | Dorothea Brandt | GER | 25.83 |