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Date: 18 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Lenny Krayzelburg | USA | 53.72 |
| 2 | Matt Welsh | AUS | 54.07 |
| 3 | Stev Theloke | GER | 54.82 |
| 4 | Josh Watson | AUS | 55.01 |
| 5 | Bartosz Kizierowski | POL | 55.04 |
| 6 | Neil Walker | USA | 55.14 |
| 7 | Steffen Driesen | GER | 55.27 |
| 8 | Eithan Urbach | ISR | 55.74 |