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Date: 22 September 2000
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Gary Hall Jr. | USA | 21.98 |
| 1 | Anthony Ervin | USA | 21.98 |
| 3 | Pieter van den Hoogenband | NED | 22.03 |
| 4 | Lorenzo Vismara | ITA | 22.11 |
| 5 | Bartosz Kizierowski | POL | 22.22 |
| 6 | Alexander Popov | RUS | 22.24 |
| 7 | Mark Foster | GBR | 22.41 |
| 8 | Oleksandr Volynets | UKR | 22.51 |