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Date: 21 September 2000
Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Gary Hall Jr. | USA | 22.07 |
| 2 | Anthony Ervin | USA | 22.13 |
| 3 | Mark Foster | GBR | 22.32 |
| 4 | Brendon Dedekind | RSA | 22.39 |
| 5 | Chris Fydler | AUS | 22.41 |
| 6 | Johan Kenkhuis | NED | 22.47 |
| 7 | Stefan Nystrand | SWE | 22.49 |
| 8 | Ricardo Busquets Healy | PUR | 22.51 |
| Rank | Name | Nation | Time |
| 1 | Pieter van den Hoogenband | NED | 22.11 |
| 2 | Alexander Popov | RUS | 22.17 |
| 3 | Lorenzo Vismara | ITA | 22.30 |
| 4 | Bartosz Kizierowski | POL | 22.35 |
| 5 | Oleksandr Volynets | UKR | 22.36 |
| 6 | Roland Schoeman | RSA | 22.41 |
| 7 | Brett Hawke | AUS | 22.49 |
| 8 | Denis Pimankov | RUS | 22.89 |