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Date: 16 September 2000
| Rank | Name | Nation | Time |
| 1 | Felicia Ballanger | FRA | 34.140 |
| 2 | Michelle Ferris | AUS | 34.696 |
| 3 | Jiang Cuihua | CHN | 34.768 |
| 4 | Wang Yan | CHN | 35.013 |
| 5 | Chris Witty | USA | 35.230 |
| 6 | Ulrike Weichelt | GER | 35.315 |
| 7 | Kathrin Freitag | GER | 35.473 |
| 8 | Tanya Dubnicoff | CAN | 35.486 |
| 9 | Iryna Yanovych | UKR | 35.512 |
| 10 | Daniela Larreal | VEN | 35.728 |
| 11 | Magali Faure-Humbert | FRA | 35.766 |
| 12 | Szilvia Noemi Szabolcsi | HUN | 35.778 |
| 13 | Lori-Ann Muenzer | CAN | 35.846 |
| 14 | Lyndelle Higginson | AUS | 35.859 |
| 15 | Oxana Grichina | RUS | 36.169 |
| 16 | Fiona Ramage | NZL | 36.536 |
| 17 | Mira Kasslin | FIN | 37.145 |