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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Deon Hemmings | JAM | 54.00 |
| 2 | Daimi Pernia | CUB | 54.92 |
| 3 | Tasha Danvers | GBR | 54.95 |
| 4 | Ulrike Urbansky | GER | 55.23 |
| 5 | Heike Meissner | GER | 55.73 |
| 6 | Kim Batten | USA | 55.73 |
| 7 | Natalya Torshina | KAZ | 56.22 |
| 8 | Yuliya Nosova | RUS | 56.58 |
| Rank | Name | Nation | Time |
| 1 | Irina Privalova | RUS | 54.02 |
| 2 | Nezha Bidouane | MAR | 54.19 |
| 3 | Tetyana Tereshchuk-Antipova | UKR | 54.25 |
| 4 | Ionela Tirlea | ROU | 54.70 |
| 5 | Gudrun Arnardottir | ISL | 54.82 |
| 6 | Sandra Glover | USA | 54.98 |
| 7 | Andrea Blackett | BAR | 55.30 |
| 8 | Catherine Scott-Pomales | JAM | 55.78 |