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Date: 22 July 1996
Summary
Final 1
| Rank | Name | Nation | Time |
| 1 | Alexander Popov | RUS | 48.74 |
| 2 | Gary Hall Jr. | USA | 48.81 |
| 3 | Gustavo Borges | BRA | 49.02 |
| 4 | Pieter van den Hoogenband | NED | 49.13 |
| 5 | Fernando Scherer | BRA | 49.57 |
| 6 | Pavlo Khnykin | UKR | 49.65 |
| 7 | Ricardo Busquets Healy | PUR | 49.68 |
| 8 | Francisco Sanchez | VEN | 49.84 |
| Rank | Name | Nation | Time |
| 1 | Jon Olsen | USA | 49.80 |
| 2 | Christian Troeger | GER | 49.90 |
| 3 | Bjorn Zikarsky | GER | 49.91 |
| 4 | Sion Brinn | JAM | 50.09 |
| 5 | Chris Fydler | AUS | 50.31 |
| 6 | Rostyslav Svanidze | UKR | 50.43 |
| 7 | Stephen Clarke | CAN | 50.45 |
| 8 | Bartosz Kizierowski | POL | 50.51 |