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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | Deon Hemmings | JAM | 52.99 |
| 2 | Tonja Buford-Bailey | USA | 53.38 |
| 3 | Heike Meissner | GER | 54.27 |
| 4 | Ionela Tirlea | ROU | 54.41 |
| 5 | Susan Smith | IRL | 54.93 |
| 6 | Ann Mercken | BEL | 54.95 |
| 7 | Tanya Ledovskaya | BLR | 54.99 |
| 8 | Tetyana Tereshchuk | UKR | 55.34 |
| Rank | Name | Nation | Time |
| 1 | Kim Batten | USA | 53.65 |
| 2 | Silvia Rieger | GER | 54.27 |
| 3 | Rosey Edeh | CAN | 54.49 |
| 4 | Debbie-Ann Parris | JAM | 54.72 |
| 5 | Sandra Farmer-Patrick | USA | 54.73 |
| 6 | Gudrun Arnardottir | ISL | 54.81 |
| 7 | Michele Schenk | SUI | 55.96 |
| Did not finish | |||
| Sally Gunnell | GBR | ||