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Summary
Semi-final 1
| Rank | Name | Nation | Time |
| 1 | John Carlos | USA | 20.1 |
| 2 | Peter Norman | AUS | 20.2 |
| 3 | Michael Fray | JAM | 20.4 |
| 4 | Roger Bambuck | FRA | 20.4 |
| 5 | Ivan Moreno | CHI | 20.8 |
| 6 | Richard Steane | GBR | 20.8 |
| 7 | Nickolay Ivanov | URS | 20.8 |
| 8 | Fernando Acevedo | PER | 20.8 |
| Rank | Name | Nation | Time |
| 1 | Tommie Smith | USA | 20.1 |
| 2 | Edwin Roberts | TTO | 20.4 |
| 3 | Larry Questad | USA | 20.4 |
| 4 | Joachim Eigenherr | FRG | 20.4 |
| 5 | Gregory Lewis | AUS | 20.5 |
| 6 | Edward Romanowski | POL | 20.7 |
| 7 | Ralph Banthorpe | GBR | 20.8 |
| 8 | Manikavagasam Jegathesan | MAS | 21.0 |