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Date: 14 July 1900 / 15 July 1900
Heats
Final
| Rank | Name | Nation | Time |
| 1 | Maxie Long | USA | 49.4 |
| 2 | Bill Holland | USA | |
| 3 | Ernst Schultz | DEN | |
| 4 | William Moloney | USA | |
| 4 | Dixon Boardman | USA | |
| 4 | Harry Lee | USA | |
| Heats | |||
| 7 | Harvey Lord | USA | |
| 7 | Charles-Robert Faidide | FRA | |
| 7 | Henry Slack | USA | |
| 10 | Georges Clement | FRA | |
| 10 | Pal Koppan | HUN | |
| 10 | Walter Drumheller | USA | |
| 10 | Yngvar Bryn | NOR | |
| 10 | Zoltan Speidl | HUN | |
| 10 | Umberto Colombo | ITA | |