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Date: 6 April 1896 / 7 April 1896
Heats
Final
| Rank | Name | Nation | Time |
| 1 | Tom Burke | USA | 54.2 |
| 2 | Herbert Jamison | USA | 55.2 |
| 3 | Charles Gmelin | GBR | |
| 4 | Fritz Hofmann | GER | |
| Heats | |||
| 5 | Frantz Reichel | FRA | |
| 6 | Alphonse Grisel | FRA | |
| 6 | Kurt Doerry | GER | |